Table 2.

Calculated Fluorescence Internal Quenching Correction Factor for each SNAPtide concentration at each temperature used for enzyme kinetics studies. Standard deviations of the mean for the triplicate samples are shown.

SNAPtide Concentration (μM)	25°C	30°C	37°C	40°C	45°C	50°C
0.00	1.00	1.00	1.00	1.00	1.00	1.00
1.00	0.982 ± 0.001	0.982 ± 0.002	0.984 ± 0.001	0.983 ± 0.002	0.983 ± 0.002	0.984 ± 0.004
1.50	0.960 ± 0.002	0.962 ± 0.003	0.963 ± 0.001	0.964 ± 0.003	0.962 ± 0.002	0.961 ± 0.005
2.00	0.947 ± 0.003	0.949 ± 0.003	0.951 ± 0.001	0.952 ± 0.004	0.949 ± 0.003	0.948 ± 0.007
4.00	0.895 ± 0.005	0.898 ± 0.001	0.902 ± 0.003	0.903 ± 0.009	0.898 ± 0.006	0.897 ± 0.014
8.00	0.789 ± 0.011	0.796 ± 0.013	0.805 ± 0.006	0.806 ± 0.018	0.796 ± 0.012	0.794 ± 0.029
10.0	0.736 ± 0.013	0.745 ± 0.017	0.756 ± 0.007	0.758 ± 0.022	0.745 ± 0.015	0.742 ± 0.036