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. Author manuscript; available in PMC: 2013 Aug 13.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2007 Oct;48(10):4785–4794. doi: 10.1167/iovs.07-0343

Fig. 2. Neuroprotective effect of (+)-PTZ on glutamate (Glu)-induced 1°GC death.

Fig. 2

(A) Photomicrographs of the live/dead assay in 1°GCs exposed 6 h to Neurobasal medium with no additional Glu (control) or to Neurobasal containing 10 µM, 25 µM and 50 µM Glu. Living cells fluoresce green; dead cells fluoresce red. (B) Quantitation of live 1°GCs following exposure to Glu (10, 25 and 50 µM) for 6 h, (*, significantly different from control and 10 µM Glu, p<0.001); (C) 1°GCs were pretreated with (+)-PTZ (0.5, 1, 3 µM) for 1 h and then co-exposed to 25 µM Glu and (+)-PTZ (0.5, 1, 3 µM) for an additional 16 h. Cells were subjected to the live/dead assay to determine viability. (**, significantly different from control cells (not treated with Glu) and from cells exposed to Glu and (+)-PTZ, p<0.001). Data in panels B and C are expressed as the mean and S.E. of the ratio of living cells to the total number of cells; data were normalized to the control value which was considered 100%, n = 10