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. Author manuscript; available in PMC: 2013 Aug 13.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2007 Oct;48(10):4785–4794. doi: 10.1167/iovs.07-0343

Fig. 3. Assessment of the number of TUNEL-positive 1°GCs following 6 h incubation with Glu in the absence or presence of (+)-pentazocine ((+)-PTZ).

Fig. 3

1°GCs were pre-treated for 1 h with or without (+)-PTZ (0.5 or 1 µM) followed by co-incubation for 6 h with Glu (25 µM) and (+)-PTZ (0.5 or 1 µM); the number of TUNEL-positive cells was determined using the ApopTag fluorescein method. Data are expressed as the mean and S.E. of the ratio of dead/dying (TUNEL-positive) cells to the total number of cells, n = 10 (*, significantly different from untreated control or vehicle (DMSO) control at p<0.001; **, significantly different from treatment with 25 µM Glu alone (without (+)-(+)-PTZ)).