Table 5. Key physiological parameters related to nitrogen metabolism: uptake rate of organic and inorganic nitrogen sources expressed as N-mmol gDW-1 h-1; incorporation of total, organic and inorganic nitrogen normalized by glucose uptake (N-mmol C-mol-1 Glucose); percentage of organic and inorganic nitrogen incorporated; contribution of each amino acid (in percentage) to the incorporation of organic nitrogen.
240 g L-1 Glucose |
280 g L-1 Glucose |
|||
---|---|---|---|---|
N-mmol gDW-1 h-1 | 16 °C | 28 °C | 16 °C | 28 °C |
Total | 0.70 ± 0.01a | 1.74 ± 0.05b | 0.70 ± 0.01a | 1.58 ± 0.04b |
Organic | 0.57 ± 0.01a | 1.09 ± 0.02b | 0.59 ± 0.01a | 0.98 ± 0.02b |
Inorganic | 0.13 ± 0.01a | 0.64 ± 0.02b | 0.11 ± 0.01a | 0.60 ± 0.03b |
N-mmol C-mol-1 Glucose | ||||
Total | 24.0 ± 0.6 | 19.9 ± 2.1 | 30.2 ± 1.5 | 21.5 ± 1.6 |
Organic | 19.5 ± 0.5 | 12.6 ± 1.4 | 25.5 ± 1.3 | 13.3 ± 1.0 |
Inorganic | 4.5 ± 0.3 | 7.4 ± 0.8 | 4.7 ± 0.5 | 8.2 ± 0.7 |
% of N incorporated | ||||
Organic | 81.3 ± 1.84a | 63.0 ± 1.87b | 84.4 ± 2.13a | 61.9 ± 2.02b |
Inorganic | 18.7 ± 1.45a | 37.0 ± 1.44b | 15.6 ± 1.56a | 38.1 ± 2.13b |
Amino acid contribution to organic N uptake (%) | ||||
Alanine | 10.7 | 10.0 | 10.6 | 9.2 |
Arginine | 36.7 | 41.9 | 37.8 | 42.2 |
Aspartate | 4.9 | 6.2 | 5.5 | 6.7 |
Cysteine | 0.4 | 1.2 | 0.3 | 1.0 |
Glutamine | 14.3 | 6.6 | 15.1 | 6.3 |
Glutamate | 2.2 | 3.3 | 1.1 | 3.2 |
Glycine | 0.5 | 0.5 | 0.5 | 0.4 |
Histidine | 0.3 | 0.1 | 0.1 | 0.2 |
Isoleucine | 2.0 | 2.3 | 1.7 | 2.3 |
Leucine | 3.6 | 3.7 | 3.5 | 3.8 |
Lysine | 3.4 | 3.1 | 4.4 | 3.8 |
Methionine | 1.6 | 1.7 | 1.3 | 1.7 |
Phenylalanine | 1.3 | 1.9 | 1.1 | 1.9 |
Serine | 8.1 | 7.1 | 7.7 | 7.0 |
Threonine | 6.2 | 6.0 | 5.6 | 5.9 |
Tryptophan | 1.2 | 1.6 | 1.1 | 1.2 |
Tyrosine | 0.3 | 0.5 | 0.2 | 0.3 |
Valine | 2.5 | 2.3 | 2.2 | 2.7 |
Different superscripts indicate statistically significant differences between values (p < 0.05).