FIG. 3.
Effect of TNFR deficiency on the expansion of virus-specific CD8 T cells during a chronic LCMV infection. Groups of +/+, p55−/−, p75−/−, and DKO mice were infected with LCMV-clone 13. On the eighth day after infection, splenocytes were stained with anti-CD8, anti-CD44, and MHC-I tetramers (Db) loaded with the CTL epitope peptides NP396 or GP33 and analyzed by flow cytometry. (A) Dot plots gated on total CD8 T cells; the numbers represent percentages of epitope-specific CD8 T cells among splenocytes, and numbers in parentheses are percentages of epitope-specific CD8 T cells per total CD8 T cells in the spleen. (B) Total number of LCMV-specific CD8 T cells in the spleen; the data are the means of three to nine mice per group ± the standard deviation.