FIG. 4.

Effect of TNFR deficiency on the expansion of virus-specific IFN-γ-producing CD8 cells during a chronic LCMV infection. On the eighth day after infection with LCMV-clone 13, splenocytes from +/+, p55^−/−, p75^−/−, and DKO mice were stimulated in vitro with the indicated LCMV CTL epitope peptides. Following in vitro stimulation, cells were stained for cell surface CD8 and intracellular IFN-γ. The number of IFN-γ-producing CD8 T cells was determined by flow cytometry. (A) Dot plots gated on total splenocytes; the numbers are the percentages of IFN-γ-producing CD8 T cells per total splenocytes. (B) Total number of epitope-specific IFN-γ-producing CD8 T cells; the data are the means of three to nine mice per group ± the standard deviation.