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MHC-I tetramers were used to study the kinetics of LCMV-specific CD8 T-cell responses in chronically infected TNFR-deficient mice. Groups of +/+, p55−/−, p75−/−, and DKO mice were infected with LCMV-clone 13, and the numbers of CD8 T cells specific to the two immunodominant epitopes NP396 (A) and GP33 (B) in the spleen were determined by staining with MHC-I tetramers. The data are the means of at least three mice per group at each time point ± the standard deviation.
