Figure 4. High-fat diet (HFD) for three days increased the pool of astroglial cells in the hypothalamus.

Mice were kept on standard or HFD for 3 days and were then euthanized. Brain sections were examined after immunostaining of Ki67-expressing proliferative cells. (A) Representative images showing Ki67-positive proliferative cells (in red) detected in the hypothalamus and phenotyped by the following markers (in green): GFAP (astrocytes) and Iba-1 (microglia). (B) Quantification of double-labeled cells in hypothalamus of mice fed standard diet or HFD. Data are means ± SEM (n = 4–5 for each group). Groups were compared using unpaired t test. * and ** denotes p≤0.05 and p≤0.01, respectively; 3V: third ventricle. HFD: high-fat diet; STD: standard diet.