Figure 6. Effects of Ag diet on the development of tumor xenografts. (А) Left, dynamics of human colon tumor growth in control (◊), Ag(+) (■), and Ag(P) (●) mice. Ordinate, weight of tumors, g; abscissa, days. Average weights for five mice in the control group, 6 Ag(+) mice and 6 Ag(P) mice are shown; right, weights of the 24-d-old tumors in the individual mice. (B) Serum oxidase activity (upper) and Cp protein (lower) detected in the same mice. (C) Serum oxidase activity (upper) and Cp protein (lower) detected in B16 melanoma-bearing mice. For oxidase activity measurements, serum aliquots (1.0 µl) were resolved by 8% native PAGE, and the gel was stained with o-dianisidine; the bands are dark orange. For Cp protein measurements, serum aliquots (0.5 µl) were fractioned by 8% native PAGE followed by immunoblotting with antibodies to human Cp. (D) Copper and silver content in the livers and tumors of the mice bearing implanted tumors. *P < 0.05, **P < 0.005, ***P < 0.0001.