Figure 1. (A) PCR genotyping of Cables1 and Apc. In the upper panel, lanes 1, 2, and 3 represent the Cables1 wild-type, heterozygous, and homozygous genotypes, respectively. WT, wild-type fragment (~300 bp); KO, knockout fragment (~200 bp). In the lower panel, lanes 1 and 2 represent the Apc^Min/+ and Apc wild type genotypes, respectively. WT fragment (~600 bp); Apc^Min fragment (~340 bp). (B) Intact nuclear Cables1 expression by immunohistochemistry in Cables1^+/+ Apc^Min/+ mouse intestinal epithelial cells. (C) Loss of nuclear Cables1 expression by immunohistochemistry in Cables1^−/−Apc^Min/+ mouse intestinal epithelial cells. A few inflammatory cells retain Cables1 expression.