Table 1. The chart represents visual estimations of cell size, transfection strength, appearance of focal adhesions, filopodia, lamellipodia, cell mobility, and displacement over the period imaged.

Mutant	Transfection strength	Focal Adhesion	Filopodia	Lamellipodia	Mobility	Displacement
WT	+ +	+	+ +	+ +	+ +	+
P46S	+ + +	+	+ +	+ +	+ +	+
P52L	+ +	+ +	+ +	+ + +	+ + +	+ +
G105D	+ +	+	+	+ +	+ + +	+
A127T	+ +	+ + +	+ + +	+ + +	+ + +	+ + +
P233L	+ +	+ + +	+ +	+ + +	+ + +	+ + +
T255I	+ +	+	+ +	+ + +	+ +	-
D399N	+ + +	-	+	+ +	+ +	+
E423K	+	+	+	+	+ +	+
P487L	+ +	+ + +	+ +	+ + +	+ +	-
K506R	+ + +	-	+	+ +	+ + +	+

At least 6–13 cells were tracked for each mutant.