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. 2001 Jul 17;98(15):8227–8234. doi: 10.1073/pnas.111008698

Figure 2.

Figure 2

Stimulation of RuvC cleavage of forked DNA requires RecG helicase activity. (A) Schematic representation of the junction structures that χSma DNA adopts in vitro. The junction is located within a 300-bp region of homology flanked by heterologous duplex arms of 0.8–1.6 kb. χSma preferentially adopts a fork structure, but the junction point has the ability to branch migrate within the homologous core to form a Holliday junction. RecG has been proposed to unwind the fork structure to generate a Holliday junction that can then be cleaved symmetrically (arrows) by junction-specific endonucleases such as RuvC. (B) Cleavage of χSma fork DNA by RuvC and the effects on cleavage of the presence or absence of RecG helicase activity. Reactions contained 5 mM ATP or ATPγS, 10 nM RuvC, 1 nM RecG, and 1 nM RecGK302A as indicated.