FIG. 1.

Enhanced GFP expression by RUB SP in trans; GFP expression in cotransfected cells. Vero cells were transfected with RUBrep/GFP (A to D) or RUBrep/GFP_ΔNotI (E to H) in the absence (MI) (A, E, and I) or presence (Inf) (B, F, and J) of helper virus or cotransfected with P200 RNA (C, G, and K) or SP RNA (D, H, and L). Infections were done with RUB F-therien at a multiplicity of infection of 1, and transfection was done at 24 h postinfection. GFP expression was examined 48 h after transfection.