Table 4. Results of organotypic spinal cord co-cultures with reconstructed ventral root incubated with 100 µM minocycline in comparison with control slices.

DIV	number of anti-pan-NF stained motor neurons	anti-pan-NF stained area in %	anti-GFAP stained area in %	anti-GFAP fluorescence intensity in arbitrary units
1	2.6 ± 3.1 vs. 35.3 ± 13.6*,ratio 0.1 ± 0.1, n=6, p=0.0016	13.1 ± 3.2 vs. 1.2 ± 0.7*,ratio 12.7 ± 4.5, n=6, p=0.0001	29.7 ± 14.8 vs. 28.7 ± 13.1, ratio 1.0 ± 0.1, n=4, p=0.45	11.1 ± 3.8 vs. 10.5 ± 3.1, ratio 1.0 ± 0.1, n=4, p=0.2531
4	30.3 ± 13.6 vs. 55.6 ± 19.5*,ratio 0.6 ± 0.2, n=6, p=0.0063	3.9 ± 2.4 vs. 2.9 ± 2.3, ratio 1.9 ± 1.8, n=6, p=0.26	33.7 ± 8.9 vs. 36.8 ± 7.9, ratio 0.9 ± 0.1, n=6, p=0.06	11.5 ± 2.0 vs. 13.4 ± 2.1*,ratio 0.9 ± 0.0, n=6, p<0.0001

All values are given as mean ± SD. day in vitro (DIV), versus (vs.), number of samples (n), error level (p), * statistical significance p<0.05.