Figure 5. BMPRII suppresses Smad1 signaling in a dose- and tail domain-dependent manner.

A) BMPRII suppresses BRE₂-luciferase activity in a dose-dependent manner. PC3-M cells were transfected with indicated siRNA and BRE₂-luciferase and Renilla luciferase constructs as in Figure 2B. Wild type BMPRII was co-transfected over a range of concentrations; x-axis displays ng of plasmid. Luciferase assay performed as in Figure 2B. Data represent mean ± SD of a single representative experiment (N = 2 replicates), repeated 3 times (N = 2 replicates) with similar results. *, p≤0.05 compared to 0/siNeg. # p≤0.05 compared to 0/siBMPR2. B) Experiment performed as in A, except that tail domain deleted (Δtail) – not wild type – BMPRII was expressed over a range of concentrations. Data represent mean ± SD from one representative of two experiments, each in replicates of N = 2. *, p≤0.05 compared to 0/siNeg.