Figure 6. BMPRII suppresses ActRIIA-mediated Smad1 activity.

PC3-M cells were transfected with BRE₂-luciferase, Renilla luciferase, and indicated plasmid DNA with or without indicated siRNA. siRNA lanes marked with a hyphen were transfected with non-targeting siRNA. Two days later cells were lysed and luciferase activity was assessed as in Figure 2B. A) Increased BRE₂-luciferase activity upon silencing BMPRII is mediated by ActRIIA. Neg  =  non-targeting siRNA. 2A  =  siActRIIA. 2B  =  siActRIIB. Data represent mean ± SD of a single representative experiment (N = 2 replicates), repeated twice (N = 2 replicates) with similar results. *, p≤0.05 for indicated comparison. B) BMPRII-mediated suppression of BRE₂-luciferase activity is dependent on ActRIIA expression. BMPRII construct abbreviations as in Figure 4. Data represent mean ± SD of a single representative experiment (N = 2 replicates), repeated 3 times (N = 2 replicates) with similar results. *, p≤0.05 for indicated comparison. C) BMPRII suppresses signaling from ActRIIA. Data represent mean ± SD from one representative experiment (N = 2 replicates) of three repeated separately (N = 2). *, p<0.05 for indicated comparison.