Figure 5. Kinetics of ATP hydrolysis by mutant α₃β₃γ^S3Cε^133C.

ATPase activities of α₃β₃γ^S3Cε^133C (S3C), and α₃β₃γ^WTε^133C (WT) at 2 mM ATP were determined. The α₃β₃γε complex of was added to 3 nM at the times indicated by the first arrowheads. (A) DTT (50 mM) and LDAO (0.1%) were added at the times indicated by the second and third arrowheads, respectively. (B) The order of addition of DTT and LDAO was reversed.