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. Author manuscript; available in PMC: 2014 Aug 1.
Published in final edited form as: Fungal Genet Biol. 2013 Jun 5;57:58–75. doi: 10.1016/j.fgb.2013.05.006

Figure 5. Rac paralogs play only a minor role in actin polarization.

Figure 5

A) Wild type, rac1Δ, and rac2Δ cells were grown in liquid YPD medium for 4 hours at 30 or 37°C and then stained for actin (Rhodamine-conjugated Phalloidin) and DNA (DAPI). Actin was observed polarized to bud tips in a majority of wild type and mutant cells (Scale bar = 5 μm). B) Wild type and the indicated mutant cells were grown in the presence of varying concentrations of the actin inhibitor Latrunculin B for 48 hours at 30°C. Bars indicate minimum inhibitory concentration (duplicate wells, n=4).