TABLE 1.
IFA detection of lytic and latent WT KSHV infection of epithelial cells
| Cell type | Virus | Detection of lytic virus/detection of latent virus at indicated hpia
|
||||
|---|---|---|---|---|---|---|
| 12 | 24 | 48 | 72 | 96 | ||
| MeWo | BCBL | −/+ | ±/++ | −/+ | −/− | −/− |
| WT | −/+ | −/++ | −/± | −/− | −/− | |
| A293T | BCBL | ND | +/± | +/± | ±/− | ±/− |
| WT | ND | +/± | ++/± | +++/− | ++++/− | |
| P-EPI | BCBL | ND | ++/++ | +/++ | +/+ | −/± |
| WT | ND | ++/++ | +/++ | +++/+ | ++++/+ | |
| T-EPI | BCBL | ND | ++/++ | +/++ | +/+ | −/± |
| WT | ND | ++/++ | +/++ | ++/+ | +++/+ | |
a
Lytic virus was measured by counting the K8.1-positive cells in the monolayer. −, not detectable; ±, <1%; +, 1 to 2%; ++, 3 to 5%; +++, 6 to 10%; ++++, >10%. Latent virus was measured by counting the LANA-positive cells in the monolayer. −, not detectable; ±, <1%; +, 1 to 5%; ++, 6 to 10%. ND, not done.