Figure 1. Effect of BPA exposure on sex steroid hormone production over time.
After exposure of antral follicles to DMSO control or BPA (1–100μg/mL) for 24–96hrs in vitro, the media were collected at each time-point, pooled per treatment group, and subjected to enzyme-linked immunosorbent assays (ELISA) for progesterone, androstenedione, testosterone, and estradiol. The graphs represent means ± SEMs from at least three separate experiments. Asterisks (*) indicate p≤ 0.05 from DMSO control.