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. Author manuscript; available in PMC: 2014 Sep 1.
Published in final edited form as: Toxicol Appl Pharmacol. 2013 May 22;271(2):249–256. doi: 10.1016/j.taap.2013.04.028

Figure 5. Effect of BPA removal on expression of StAR and Cyp11a1 over time.

Figure 5

After exposure of antral follicles to DMSO control or BPA (1 or 10μg/mL) for 20h in vitro, the media were removed from follicles exposed to BPA (1 or 10μg/mL) and replaced with freshly prepared supplemented media with DMSO. Follicles were then cultured for 4, 28, 52, or 76 more hours until total time in culture was 24h, 48h, 72h, or 96h. At each time-point, follicles were collected and subjected to qPCR for analysis for A) StAR and B) Cyp11a1 mRNA expression levels. All values were normalized to beta-actin as a loading control. The graphs represent means ± SEMs from three separate experiments. Asterisk (*) indicates p≤ 0.05 from DMSO control.