Figure 5. Analysis pmGENIE-4 mediated transposition. 0.5 × 10⁵ HEK293T cells were transfected with 400 ng of pmhyGENIE-3 EGFP, pmhyGENIE-4 EGFP, pmhyGENIE-4-RNA-OUT EGFP or pmGENIE-3 ∆-pB. Transposition activity was measured by counting methylene blue stained, hygromycin-resistant colonies after a three-week selection period. Data are shown as mean values with SD (n = 3).