Figure 1. HER1104 displays an uncommon small plasmid profile. Plasmids under 50 kb in size were extracted from A. salmonicida A449, 01-B526, and HER1104 and were digested with EcoRI. The digested DNA samples were separated by pulsed field gel electrophoresis. The three strains tested contained pAsa1, pAsa2, and pAsa3. It was already known that A449 does not possess pAsal1 while this plasmid is present in 01-B526. ^4,7 EcoRI-digested HER1104 DNA does not contain a band corresponding to pAsal1 but does contain a band corresponding to an unknown plasmid with a higher molecular weight, which was named pAsal1B after further analyses.