Table 2. Primers used in this study.
| Primer name | Sequence (5′-3′) | Ref. |
|---|---|---|
| Primers used for the walk on pAsal1B | ||
| MT5-R1 |
AAGTCCTCGATCTCGTCCCTGATA |
This study |
| MT7-R1 |
TTGTGGAGCGTGTTTCTCGTAGCC |
This study |
| MT9-R1 |
AGGACCGACAACCAGAACTTTGCC |
This study |
| MT11-F1 |
TTCATCTACTGGCACCATGAGCCT |
This study |
| MT13-R1 |
GTTTGATACATCCAATGGGAAGGG |
This study |
| MT15-F1 |
TGGCCTATCGCCATATCCAACACA |
This study |
| MT18-R2 |
TGTTGGATATGGCGATAGGCCAGT |
This study |
| MT19-R1 |
CTCTATGTGCTTGCACCTGGCTAA |
This study |
| MT21-F1 |
CTCACGTCATCTCGTTTCTAACT |
This study |
| MT22-R1 |
GTCACTGAAGGGAGAATCGATGAG |
This study |
| MT23-F1 |
CGGTCAGTTTCAGGTTTCTCAGG |
This study |
| MT24-R1 |
GTCCTTACAAGCCCAAGGACAAG |
This study |
| MT25-F1 |
TGCGTTAAGGATGATTCTGGTGAA |
This study |
| MT26-R1 |
GACCAATGTGAACGCTCATTCC |
This study |
| MT27-F1 |
CTTGCTCGATCTGATCCCATATCC |
This study |
| MT28-R1 |
GATACAGCAGGCTTCAAAGAAGT |
This study |
| MT29-F1 |
TCTTTAGTTGCCTCTTGGATTG |
This study |
| MT30-R1 |
CGGAATGAAGTGTGATGTTCTTG |
This study |
| MT31-F1 |
TTGTGTTGGATATGGCGATAGG |
This study |
| MT32-R1 |
TTAGAGAGTACTGGCGGCCTAA |
This study |
| MT33-F1 |
CTTCCAGCAGTTCCCGATTTAT |
This study |
| MT33-F2 |
TAACGTTACTCTCCTGCTGTTTG |
This study |
| MT34-F1 |
CTATCGATGCCAATCAGCAAAC |
This study |
| MT34-F2 |
ATCGATGCCAATCAGCAAAC |
This study |
| MT35-R1 |
CATGGATGATCGGTATGGGAAATA |
This study |
| MT35-R2 |
GGTCAACTGGCAAGGATAGAG |
This study |
| MT36-R1 |
CAAACCAGCTTCCCATCAGTAA |
This study |
| MT36-R2 |
CATATCCAGCTCATGCCAAAC |
This study |
| Primers used for the PCR analyses | ||
| To test the presence of the aopP gene | ||
| pAsal1 F |
TAACATGGGTGAGTCAGGA |
4 |
| pAsal1 R |
TGCATGTTTGTAAAAAGTAGGTG |
4 |
| To test the presence of pRAS3 | ||
| pRAS3deb-F |
CATGAGCATTGCGGTAGCACTCAA |
This study |
| pRAS3deb-R |
TCGCTTGCGGGAACTTCTCATACT |
This study |
| pRAS3mil-F |
GCGGGTCGAACAAATCTGGTTCTT |
This study |
| pRAS3mil-R |
ACTGTTGGCTGTGCTCAAACATCC |
This study |
| To test the rearrangement status of pAsa5 | ||
|
tapA F |
ACATGAAGAAGCAATCAGGC |
30 |
|
tapA R |
AGAGGTCATGCGTTAGCAG |
30 |
|
traC F |
TGCACTATCCCCAGCTATCC |
8 |
|
traC R |
TCGGTAATCGCGGTCTTGTC |
8 |
|
exsD F |
AGAAGTGATCCTGACCCAAGGCAA |
8 |
|
exsD R |
TTGCAACGACTGTTGCCAAGAACC |
8 |
|
resD F |
TCAGAAACTTGGCCATCGCTCACA |
8 |
|
resD R |
TGATGTGCAGATTTCCCTGGAGACA |
8 |
| 11AF |
AATAGGTGTCGCAAGCTGGGTTGA |
7 |
| 11CR | AACTGGCAAGGATAGAGCTGCTGA | 7 |