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. 2001 Jul 17;98(15):8235–8240. doi: 10.1073/pnas.121007798

Figure 2.

Figure 2

Both RusA and RuvC can cleave ERIs. Standard oriC replication reactions containing either RusA (lanes 2–5, concentration varied by a factor of 10 in each lane, left to right), RuvC (lanes 7–10, concentration varied by a factor of 10 in each lane, left to right), or having no addition (lanes 1 and 6) were incubated at 37°C for 5 min. The reactions then were terminated by the addition of EDTA, and the DNA products then were analyzed by electrophoresis through either a neutral 1% agarose gel (A) or a denaturing 1% alkaline agarose gel (B) as described in Materials and Methods. α, α structure; FII, form II (nicked, circular DNA). The mobility difference between the ERI and the α structure is subtle and can be seen more clearly in Figs. 5–7.