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. 2001 Jul 17;98(15):8235–8240. doi: 10.1073/pnas.121007798

Figure 3.

Figure 3

RuvA inhibits cleavage of the ERI by RusA. Standard oriC replication reactions containing either 10 nM RusA (lane 2), 10 nM RusA and RuvA (lanes 3–5, RuvA concentration varied by a factor of 10 in each lane from left to right), 100 nM RuvA (lane 6), or having no addition (lane 1) were incubated first for 2 min at 37°C after RuvA addition and then for an additional 5 min at 37°C after RusA addition. The reactions were terminated by the addition of EDTA, and the DNA products then were analyzed by electrophoresis through a neutral 1% agarose gel.