Figure 6.

RecG stimulates RusA-catalyzed cleavage of the ERI. Inactivated ERI was formed as in the legend to Fig. 4 except that six reaction mixtures were pooled. Fresh ATP was added to this pool to a concentration of 2 mM. Six and three-tenths microliters of the inactivated ERI pool was then incubated in new reaction mixtures (7 μl) containing either no RecG or RusA (lane 1), RecG (lanes 2–4 and 6–8, concentration varied by a factor of 10 in lanes 2–4 and 6–8, left to right), and 10 nM RusA (lanes 5–8) for 5 min at 37°C. The reactions were terminated by the addition of EDTA, and the DNA products were analyzed by neutral 1% agarose gel electrophoresis.