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Cell based sensors for monitoring BDNF or ACh secretion. (a) Bescell detected BDNF release using a genetically encoded FRET sensor for protein phosphorylation. (b) In CNiFERs, activation of the M1 ACh receptor increased cytosolic Ca2+ via the Gq/inositol triphosphate (IP3) second messenger pathway. Cytosolic Ca2+ was monitored with a genetically encoded FRET Ca2+ sensor, TN-XXL.
