FIG. 3.

UV-irradiated CVB3, which is capable of receptor binding and endocytosis but not replication, fails to induce Akt phosphorylation. HeLa cells were infected with wild-type or UV-irradiated CVB3, and cell lysates were collected at 7 h postinfection (pi) and subjected to Western blotting to detect the phosphorylation of Akt on Ser-473 and Thr-308 residues. Virus attachment to specific receptor-coreceptor complex is not sufficient for Akt phosphorylation, suggesting that viral postentry replication is required for Akt activation. The results shown are representative of those from triplicate experiments.