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. 2013 Aug 13;8(8):e71734. doi: 10.1371/journal.pone.0071734

Table 1. PCR results of cetacean skin samples.

Animal code Species Sex Age Location ofstranding Sample no. Skin 18SrRNA* β-globin* DNApol# DNAtopo# PPV-DNApol# PPV-up-do# OPV-HA#
2002/262A P. phocoena F J Humberside 8 Normal +++§ +++
2002/308 P. phocoena M A Humberside 9 Normal +++ +++
2003/296 P. phocoena F J Humberside 15 Normal +++ +++
2004/307A P. phocoena M J Humberside 19 Normal +++ +++
2004/309A P. phocoena M A Humberside 20 Normal +++ +++
2004/319 P. phocoena M A Humberside 21 Normal +++ +++
2007/85,1 P. phocoena M J Humberside 25 Normal +++ +++
2007/124 P. phocoena M A Northumberland 2 Normal +++ +++
1998/200 S. coeruleoalba F A Avon 6 Normal +++ +++
2002/178 P. phocoena M SA Gwynedd 29 Lesion ND +++ ND ND ND
2002/382 P. phocoena M J Norfolk 30 Lesion ND +++ ND ND ND
2004/342 P. phocoena M J Pembrokeshire 32 Lesion ND +++ ND ND ND
2005/286 P. phocoena F J Kent 33 Lesion ND +++ ND ND ND
2006/3 P. phocoena F J Ceredigion 34 Lesion ND +++ ND ND ND
2006/41 P. phocoena F J London 35 Lesion ND +++ ND ND ND
2006/228 P. phocoena F J Kent 36 Lesion ND +++ ND ND ND
2007/85,2 P. phocoena M J Humberside 39 Lesion ND +++ ND ND ND
2007/164 P. phocoena M SA Gwynedd 40 Lesion ND +++ ND ND ND
2007/224 P. phocoena M SA Carmarthenshire 38 Lesion ND +++ ND ND ND
2001/217 S. coeruleoalba F J Devon 28 Lesion ND +++ ND ND ND
2003/215,2 S. coeruleoalba F A Northumberland 31 Lesion ND +++ ND ND ND
2001/127 P. phocoena M A Humberside 7 Lesion (? T) +++ +++ ++ +
2003/271 P. phocoena F A Humberside 12 Lesion (old T) +++ +++ ++ +
13 Lesion (old T) +++ +++ ++ +
14 Lesion (old T) +++ +++ ++ ++
2003/312 P. phocoena F A Humberside 16 Lesion (T) +++ +++ ++ ++
2004/267 P. phocoena F SA Humberside 17 Lesion (T) +++ +++ ++ ++
18 Lesion (T) +++ +++ ++ +
2005/23 P. phocoena F A Kent 11 Lesion (T) +++ +++ ++ +++
2005/28 P. phocoena F J West Sussex 22 Lesion (T) +++ +++ ++ ++
2006/223 P. phocoena M J Devon 23 Lesion (old T) +++ +++ ++ +
24 Lesion (old T) +++ +++ ++ +
2006/262 P. phocoena F SA Dorset 37 Lesion (T) ND +++ ++ ++
2007/124 P. phocoena M A Northumberland 3 Lesion (T) +++ +++ +++ ++
2000/4 S. coeruleoalba F A Dorset 1 Lesion (T) +++ +++ ++ +
4 Lesion (T) +++ +++ +++ +
5 Lesion (T) +++ +++ +++ +
10 Lesion (T) +++ +++ ++
2008/94.17 D. delphis F J Cornwall 41 Lesion (T) ND +++ + +
vaccinia NA NA NA NA 26 NA +++ +++ ++ ++ +
sealpox NA NA NA NA 27 NA +++ +++ + + +

Species: P. phocoena - harbour porpoise (Phocoena phocoena), S. coeruleoalba - striped dolphin (Stenella coeruleoalba), D. delphis – short-beaked common dolphin (Delphinus delphis); Sex: F - Female, M - Male; Age: A - Adult, SA - Sub-adult, J – Juvenile, estimated by length; (T) – tattoo lesion; (? T)- dubious tattoo lesion; (old T) - old tattoo lesion; PPV – parapoxvirus; OPV – orthopoxvirus;

*

10 ng DNA used as template;

#

50 ng DNA used as template;

§

number of+indicates relative amount of PCR product; ND - not done; NA – not applicable.