Table 1. PCR results of cetacean skin samples.
Animal code | Species | Sex | Age | Location ofstranding | Sample no. | Skin | 18SrRNA* | β-globin* | DNApol# | DNAtopo# | PPV-DNApol# | PPV-up-do# | OPV-HA# |
2002/262A | P. phocoena | F | J | Humberside | 8 | Normal | +++§ | +++ | – | – | – | – | – |
2002/308 | P. phocoena | M | A | Humberside | 9 | Normal | +++ | +++ | – | – | – | – | – |
2003/296 | P. phocoena | F | J | Humberside | 15 | Normal | +++ | +++ | – | – | – | – | – |
2004/307A | P. phocoena | M | J | Humberside | 19 | Normal | +++ | +++ | – | – | – | – | – |
2004/309A | P. phocoena | M | A | Humberside | 20 | Normal | +++ | +++ | – | – | – | – | – |
2004/319 | P. phocoena | M | A | Humberside | 21 | Normal | +++ | +++ | – | – | – | – | – |
2007/85,1 | P. phocoena | M | J | Humberside | 25 | Normal | +++ | +++ | – | – | – | – | – |
2007/124 | P. phocoena | M | A | Northumberland | 2 | Normal | +++ | +++ | – | – | – | – | – |
1998/200 | S. coeruleoalba | F | A | Avon | 6 | Normal | +++ | +++ | – | – | – | – | – |
2002/178 | P. phocoena | M | SA | Gwynedd | 29 | Lesion | ND | +++ | – | – | ND | ND | ND |
2002/382 | P. phocoena | M | J | Norfolk | 30 | Lesion | ND | +++ | – | – | ND | ND | ND |
2004/342 | P. phocoena | M | J | Pembrokeshire | 32 | Lesion | ND | +++ | – | – | ND | ND | ND |
2005/286 | P. phocoena | F | J | Kent | 33 | Lesion | ND | +++ | – | – | ND | ND | ND |
2006/3 | P. phocoena | F | J | Ceredigion | 34 | Lesion | ND | +++ | – | – | ND | ND | ND |
2006/41 | P. phocoena | F | J | London | 35 | Lesion | ND | +++ | – | – | ND | ND | ND |
2006/228 | P. phocoena | F | J | Kent | 36 | Lesion | ND | +++ | – | – | ND | ND | ND |
2007/85,2 | P. phocoena | M | J | Humberside | 39 | Lesion | ND | +++ | – | – | ND | ND | ND |
2007/164 | P. phocoena | M | SA | Gwynedd | 40 | Lesion | ND | +++ | – | – | ND | ND | ND |
2007/224 | P. phocoena | M | SA | Carmarthenshire | 38 | Lesion | ND | +++ | – | – | ND | ND | ND |
2001/217 | S. coeruleoalba | F | J | Devon | 28 | Lesion | ND | +++ | – | – | ND | ND | ND |
2003/215,2 | S. coeruleoalba | F | A | Northumberland | 31 | Lesion | ND | +++ | – | – | ND | ND | ND |
2001/127 | P. phocoena | M | A | Humberside | 7 | Lesion (? T) | +++ | +++ | ++ | + | – | – | – |
2003/271 | P. phocoena | F | A | Humberside | 12 | Lesion (old T) | +++ | +++ | ++ | + | – | – | – |
13 | Lesion (old T) | +++ | +++ | ++ | + | – | – | – | |||||
14 | Lesion (old T) | +++ | +++ | ++ | ++ | – | – | – | |||||
2003/312 | P. phocoena | F | A | Humberside | 16 | Lesion (T) | +++ | +++ | ++ | ++ | – | – | – |
2004/267 | P. phocoena | F | SA | Humberside | 17 | Lesion (T) | +++ | +++ | ++ | ++ | – | – | – |
18 | Lesion (T) | +++ | +++ | ++ | + | – | – | – | |||||
2005/23 | P. phocoena | F | A | Kent | 11 | Lesion (T) | +++ | +++ | ++ | +++ | – | – | – |
2005/28 | P. phocoena | F | J | West Sussex | 22 | Lesion (T) | +++ | +++ | ++ | ++ | – | – | – |
2006/223 | P. phocoena | M | J | Devon | 23 | Lesion (old T) | +++ | +++ | ++ | + | – | – | – |
24 | Lesion (old T) | +++ | +++ | ++ | + | – | – | – | |||||
2006/262 | P. phocoena | F | SA | Dorset | 37 | Lesion (T) | ND | +++ | ++ | ++ | – | – | – |
2007/124 | P. phocoena | M | A | Northumberland | 3 | Lesion (T) | +++ | +++ | +++ | ++ | – | – | – |
2000/4 | S. coeruleoalba | F | A | Dorset | 1 | Lesion (T) | +++ | +++ | ++ | + | – | – | – |
4 | Lesion (T) | +++ | +++ | +++ | + | – | – | – | |||||
5 | Lesion (T) | +++ | +++ | +++ | + | – | – | – | |||||
10 | Lesion (T) | +++ | +++ | ++ | – | – | – | – | |||||
2008/94.17 | D. delphis | F | J | Cornwall | 41 | Lesion (T) | ND | +++ | + | + | – | – | – |
vaccinia | NA | NA | NA | NA | 26 | NA | +++ | +++ | ++ | ++ | – | – | + |
sealpox | NA | NA | NA | NA | 27 | NA | +++ | +++ | – | + | + | + | – |
Species: P. phocoena - harbour porpoise (Phocoena phocoena), S. coeruleoalba - striped dolphin (Stenella coeruleoalba), D. delphis – short-beaked common dolphin (Delphinus delphis); Sex: F - Female, M - Male; Age: A - Adult, SA - Sub-adult, J – Juvenile, estimated by length; (T) – tattoo lesion; (? T)- dubious tattoo lesion; (old T) - old tattoo lesion; PPV – parapoxvirus; OPV – orthopoxvirus;
10 ng DNA used as template;
50 ng DNA used as template;
number of+indicates relative amount of PCR product; ND - not done; NA – not applicable.