FIG. 2.

(A) Formation of foci was inhibited in cells expressing MxA protein. Nontransfected Vero cells, Vero cells expressing only the neomycin resistance gene (VN36), Vero cells expressing MxA(E645R) [VA(E645R)] or cells expressing wild-type MxA (VA9) were grown in chamber slides and infected with CCHFV at MOI of 0.01. At 12 or 24 hpi, the cells were fixed and analyzed for CCHFV NP expression by indirect immunofluorescence. (B) Monolayers of control cells (Vero E6 cells and VN36) and cells constitutively expressing MxA (VA9) were infected with CCHFV at MOI of 0.1. At 12 (a) or 24 (b) hpi, the cells were harvested and analyzed by Western blot using an anti-CCHFV NP antibody. Molecular markers are shown at the right side of the panel.