FIG. 3.

Accumulation of the CCHFV genome is blocked in cells expressing wild-type MxA. VA9 and VN36 monolayers were infected with CCHFV at an MOI of 0.01. After 12 and 24 hpi, the cells were lysed in TRIZOL reagent. Total RNA was extracted and the amounts of vRNA for the S (a) and M (b) segments were determined by a quantitative real-time PCR as described in Materials and Methods. Values given are the average of three independent experiments. Error bars indicate standard deviations.