FIG. 4.

(Color panels) Wild-type MxA colocalizes with NP of CCHFV in perinuclear regions of infected cells. VN36, VA(E645R), and VA9, seeded in chamber slides, were infected with CCHFV at an MOI of 0.01. At 24 hpi, the cells were fixed and analyzed for CCHFV NP (green) and MxA (red) under the confocal microscope. Panels at the right show the superimposition of the two images. (Black-and-white panels) MxA coimmunoprecipitated with CCHFV NP. Wild-type MxA-expressing Vero cells (VA9), a mutant form of MxA [VA(E645R)], and cells expressing only the neomycin gene were infected with CCHFV at an MOI of 0.1. (a) At 24 hpi, the cell monolayers were lysed and immunoprecipitated with an anti-CCHFV NP-specific antibody. The immunoprecipitate was analyzed for the presence of MxA by Western blot analysis using specific MxA antibodies. Molecular markers are shown on the left side of the panel. (b) Cell lysates from panel a were analyzed for the expression of MxA and CCHFV NP by Western blot analysis.