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. Author manuscript; available in PMC: 2014 Jan 1.
Published in final edited form as: Obesity (Silver Spring). 2013 Jun 11;21(7):1380–1388. doi: 10.1002/oby.20159

Figure 3. Immunohistochemical analysis of adipose tissue macrophages.

Figure 3

(A) Representative 20x fields of parametrial adipose tissue from obese (left) and control (right) NSG (top) and B6 (bottom) mice. Macrophages labeled with F4/80, calibration bar is 100 μm (see methods for staining procedures and microscope model). (B) Representative CLS from an obese NSG mouse labeled with F4/80. Photo taken at 63x magnification, calibration bar is 25 μm. (C) Quantification of macrophages found in crown like structures (CLS) in parametrial fat pads of NSG (n=6/group) and B6 (n=4/group) mice. CLSs were counted by a blinded observer in 15 fields at 20x magnification.