Figure 4.

ROS generation and cell viability in CNE1 and CNE2 cells following various treatments. (A) Curcumin (40 μM) was added to CNE1 and CNE2 cells which were incubated for 0.5-2 h. The cells were then stained with 10 μM DCFH-DA for 30 min. The levels of intracellular ROS were estimated using flow cytometry. (B) CNE1 and CNE2 cells were incubated with curcumin (40 μM) for 2 h, washed and exposed to PL at various energy densities (0.1, 0.2 and 0.4 J/cm²). The ROS levels were determined immediately. Data are the mean ± SE of three experiments. ^*P<0.01 vs. control. ROS, reactive oxygen species; DCFH-DA, 2,7-dichlorodihydrofluorescein diacetate; PL, purple light.