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. 2004 Apr;78(8):4108–4119. doi: 10.1128/JVI.78.8.4108-4119.2004

FIG. 1.

FIG. 1.

(A) BAY11 inhibition of NF-κB in LCLs. Electrophoretic mobility shift assay with cell lysates after treatment for the indicated times with DMSO (lanes 1 and 4) or 10 μM BAY11 (lanes 2, 3, 5, and 6). In lane 6, cells were treated with BAY11 for only 1 h. The asterisk indicates a nonspecific complex. (B) BAY11 slowly downregulates JNK expression in LCLs. LCLs were treated with DMSO or 6 μM BAY11 for 4, 8, or 24 h. Total cell lysates were analyzed by using JNK (top) or phospho-JNK (bottom) antibodies. (C) JNK1 expression is downregulated by ΔN-IκBα. IB4 cells that conditionally express ΔN-IκBα under TET control were induced for 1, 2, or 3 days. Total cell extracts were examined for JNK expression. (D) BAY11 augments p38 activity. Cells were treated as in panel B. Western blot analyses were performed with antibodies to p38 (top) or phospho-p38 (bottom).