Figure 2. MCAS CSCs/CICs are enriched in ALDH^Br cells.

A. Detection of ALDH^Br cells from MCAS cells. MCAS ovarian mucinous adenocarcinoma cells were stained with BAAA and analyzed. The percentage represents the ratio of ALDH^Br cells. Inhibitor indicate ALDH1 inhibitor (diethylamino- benzaldehyde (DEAB)). B. Tumor initiation of ALDH^Br cells derived from MCAS cells. 10⁴ ALDH^Br and ALDH^Low cells derived from MCAS cells were inoculated subcutaneously into the backs of NOD/SCID mice, and tumor growth was measured weekly. Data represent means ± SD. Differences between ALDH^Br and ALDH^Low cells were examined for statistical significance using Student's t-test. *P values. C. qPCR of CSC/CIC markers in MCAS SP and MP cells. Data represent means ± SD. Asterisks indicate significant difference. *P<0.05. t-test.