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. 2004 Apr;78(8):3953–3964. doi: 10.1128/JVI.78.8.3953-3964.2004

FIG. 6.

FIG. 6.

NFI protein and mRNA levels do not change following TGF-β treatment of HKc/HPV16. (A) Total NFI protein levels were determined by Western analysis. Nuclear extract (40 μg of protein) from TGF-β-sensitive HKc/HPV16 treated with and without 40 pM TGF-β for 48 h was separated on an SDS-12% polyacrylamide gel, transferred to nitrocellulose, and probed with an anti-NFI antibody (Santa Cruz). Molecular mass markers are shown on the right; arrows pointing to NFI bands are on the left. (B) mRNA expression of each of the four NFI family members (NFIA, NFIB, NFIC, and NFIX) was determined by real-time PCR. RNA was collected using RNeasy columns (Qiagen) from TGF-β-sensitive HKc/HPV16 treated with (gray) and without (black) 40 pM TGF-β for 46 h. Expression was determined using primers specific for each NFI family member, compared with a control set of primers, and expressed as fold induction. The average of two experiments for each family member is shown.