Table 1. LC Mobile Phase Gradient.

Total Time	Flow Rate	A (%)	B (%)
(min)	(:L/min)
0	250	65	35
0.25	250	65	35
1	250	55	45
3	250	45	55
8.5	250	34	66
12.5	250	28	72
15	250	18	82
16.5	250	5	95
18	250	5	95
18.1	250	65	35
21.5	250	65	35

Details of elution gradient.