Figure 1.

Antiproliferative and apoptotic effect of EEC on cancer cells. (A) H₂₂ cells (5×10⁴) were plated in 96-well culture plates. Subsequent to 24 h, the medium was changed to fresh medium and treated with saline alone or EEC at the indicated doses. Following 48 h of treatment, the cells were analyzed by MTT assay. (B) The effect of EEC on the DNA fragmentation of the H₂₂ cells. Ladders were detected by 1.5% agarose gel electrophoresis. M, 1K DNA marker; lane 1, untreated H₂₂ cells; lane 2, H₂₂ cells treated with EEC. (C) Following 30 min of incubation with DiOC6 (100 nM) for Δψm, the intracellular fluorescence intensity was measured. Results represent the mean ± SEM from three independent experiments. ^*P<0.05 vs. control; ^**P<0.01 vs. control. EEC, ethanol extract of Chaenomeles speciosa Nakai; Δψm, mitochondrial membrane potential.