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. 2013 Aug 13;8(8):e71117. doi: 10.1371/journal.pone.0071117

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© 2013 Douvaras et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Double immunofluorescent detection of BrdU (yellow) and CD31-positive blood vessels (red) in WT and Pax6^+/− mice. (A) Flattened WT cornea showing BrdU-positive, label-retaining cells (LRCs) and CD31-positive blood vessels in the limbus. (B) Flattened Pax6^+/− cornea demonstrating CD31-positive blood vessels extending from the limbus into the cornea and BrdU LRCs in the cornea. (C, D) Montages of flattened Pax6^+/− corneas showing CD31-positive blood vessels and BrdU LRCs even in the central cornea. Arrows show blood vessels with adjacent BrdU-positive cells (LRCs). For demonstration purposes the counterstain channel was deactivated. Abbreviations: L: Limbus; Co: Cornea; Yellow immunofluorescence: BrdU. Red immunofluorescence: CD31; Blue: TO-PRO3 iodide counterstain. Scale bars are 100 µm.