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. 2013 May 17;6(1):280–286. doi: 10.3892/ol.2013.1353

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Copyright © 2013, Spandidos Publications

This is an open-access article licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License. The article may be redistributed, reproduced, and reused for non-commercial purposes, provided the original source is properly cited.

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Figure 2. — Representative examples of MSP analyses of Apaf-1 and DAPK. Lanes 1 and 2 show samples of normal oral mucosa. Lanes 3–12 show samples of OSCC. Lanes M show the amplified product with primers recognizing methylated sequences. Lanes U show the amplified product with primers recognizing unmethylated sequences. MSP, methylation-specific polymerase chain reaction (PCR); Apaf-1, apoptosis protease activating factor-1; DAPK, death-associated protein kinase; OSCC, oral squamous cell carcinoma; M, methylated; UM, unmethylated.