Figure 3.

Analyses of Tca8113 tongue cells treated with DAC. (A) Cell viability analyses (MTT). (B) Cell apoptotic rate analyses (Annexin V-PI dual staining assay). (C) Cell apoptotic rate analyses (TUNEL). MSP analyses of (D) Apaf-1 and (F) DAPK. RT-PCR analyses of (F) Apaf-1 and (G) DAPK. DAC, 5-aza-2′-deoxycytidine; MSP, methylation-specific polymerase chain reaction (PCR); Apaf-1, apoptosis protease activating factor-1; DAPK, death-associated protein kinase; RT-PCR, reverse transcription-PCR.