Table II.

Specific primers used in MSP.

Gene name	Primer sequence	Length (bp)	Product size (bp)	Reference
Apaf-1
Methylation
F	5′-GAG GTG TCG TAG CGG TAT TC-3′	20	212	(3)
R	5′-CGA AAA TTA ACG AAA TAA ACG TC-3′	23
Unmethylation
F	5′-ATT TGA GGT GTT GTA GTG GTA TTT G-3′	25	221
R	5′-ACC TCC AAA AAT TAA CAA AAT AAA CAT-3′	27
DAPK
Methylation
F	5′-GGA TAG TCG GAT CGA GTT AAC GTC-3′	24	98	(8)
R	5′-CCC TCC CAA ACG CCG -3′	15
Unmethylation
F	5′-GGA GGA TAG TTG GAT TGA GTT AAT GTT-3′	27	106
R	5′-CAA ATC CCT CCC AAA CAC CAA-3′	21

MSP, methylation-specific polymerase chain reaction; Apaf-1, apoptosis protease activating factor-1, DAPK, death-associated protein kinase; F, forward; R, reverse.