Table I.

Primers for RT-PCR and annealing temperatures for individual genes.

Gene	Primer sequence	Size of product (bp)	Temperature (°C)
D6	Upstream 5′-CCT GCT CCT TGC TAC CAT AGT AGT G-3′	232	60
	Downstream 5′-CAC CAA GAC ACA ACC AAT ACG GGA G-3′
GAPDH	Upstream 5′-GGG AGC CAA AAG GGT CAT CAT CTC-3′	353	60
	Downstream 5′-CCA TGC CAG TGA GCT TCC CGT TC-3′
CCL2	Upstream 5′-ACT GAA GCT CGC ACT CTC GCC TC-3′	389	60
	Downstream 5′-TGT CTG GGG AAA GCT AGG GGA AAA T-3′
CCL4	Upstream 5′-TGT CCT GTC TCT CCT CAT GCT AGT A-3′	264	60
	Downstream 5′-GCT CAG TTC AGT TCC AGG TCA TAC A-3′
CCL5	Upstream 5′-AAG GTC TCC GCG GCA CGC CTC-3′	283	60
	Downstream 5′-ACT CTC CAT CCT AGC TCA TCT CCA AA-3′
EGF	Upstream 5′-GAC AAC TCC CCT AAG GCT TA-3′	462	55
	Downstream 5′-CAT GCA CAC GCC ACC ATT GAG GCA GTA CCC ATC GTA CGA-3′	566
bFGF	Upstream 5′-TTC CCA CCAGGC CAC TTCA-3′	212	48
	Downstream 5′-GCC GTC CAT CTT CCT TCA TAG C-3′
TGF-β	Upstream 5′-GAC TCC TGC TGC TTT CTC C-3′	531	60
	Downstream 5′-GCG GTC CAC CAT TAG CAC-3′
EGFR	Upstream 5′-CTT CTT GCA GCG ATA CAG CTC-3′	440	60
	Downstream 5′-ATG CTC CAA TAA ATT CAC TGC-3′

Bp, base pair; EGFR, epidermal growth factor receptor; bFGF, basic fibroblast growth factor; TGF-β, transforming growth factor-β.