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. 2013 May 22;305(3):C309–C322. doi: 10.1152/ajpcell.00293.2012

Fig. 1.

Fig. 1.

Confirmation of lamin A/C silencing. A: Western blot of whole cell protein extractions from control and lamin short hairpin (sh)RNA bovine aortic endothelial cells (BAECs) confirm the presence and absence of lamin A/C, respectively. Actin was used as the loading control. B: Hoechst staining of live cell nuclei showed that cells with lamin shRNA have irregularly shaped nuclei, and cells with non-targeting control shRNA had the characteristic circular nuclei. Images were taken at ×40 magnification. Scale bar = 15 μm.