Fig. 8.

Histone acetylase (HAT) and deacetylase (HDAC) proteins show altered function in the absence of lamin A/C. A: HDAC proteins in control shRNA cells showed increasing and significant activity when BAECs were exposed to either 25 μM Dex (2.19 ± 0.35-fold) or 25 dyn/cm² shear stress (2.95 ± 0.52-fold) compared with static (*P < 0.05, compared with control). Lamin shRNA BAECs showed a lesser and insignificant increase under stimulation (1.68 ± 0.25-fold for Dex, 1.42 ± 0.25-fold for shear stress) compared with static. B: HAT proteins in control shRNA BAECs showed significant increases in activity upon stimulation by Dex (1.11 ± 0.03-fold) or shear stress (1.17 ± 0.04-fold; *P < 0.05, compared with control). Lamin shRNA BAECs failed to show an identical trend, as Dex and shear stress both failed to induce significant increases in HAT activity (1.04 ± 0.04- and 0.99 ± 0.07-fold, respectively).