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Generation of CTRP3 Tg mice. A: schematic of Ctrp3 transgenic construct. FLAG-tagged Ctrp3 transgene is driven by a ubiquitous CAG promoter. B: semiquantitative RT-PCR analysis of Ctrp3 transgene expression in mouse tissues; β-actin was included as control. C: immunoblot analysis for the presence of CTRP3-FLAG protein in mouse tissues. β-Actin levels serve as loading control. WT, wild-type; Tg, Transgenic.
