Fig. 4.

Recombinant CTRP3 treatment reduces lipid accumulation in vitro. A: CTRP3 treatment reduces the accumulation of neutral lipids in rat H4IIE hepatocytes treated overnight with 200 μM palmitate and CTRP3 (5 μg/ml), as quantified by Oil Red O staining. B: CTRP3 decreases de novo lipid synthesis in H4IIE hepatocytes, as quantified by [³H]acetate incorporation. C: no change in lipid uptake as measured by [³H]palmitate uptake by H4IIE hepatocytes pretreated with vehicle or CTRP3. Values are mean fold ± SE. *P < 0.05 vs. vehicle.