Fig. 6.

Short-term administration of recombinant CTRP3 reduces hepatic triglyceride levels in diet-induced obese (DIO) mice. A: time line depicting the daily injection study. After 12 wk on a high-fat diet, wild-type DIO mice were fasted for 8 h before initial blood draw. After 72-h recovery from the initial fast (considered day 0), body weight of DIO mice was determined. CTRP3 (2 μg/g body wt) or vehicle injection was given every 24 h for the next 5 days. After the 5th injection, food was immediately removed, and after an 8-h fast animals were euthanized and liver tissues and sera were harvested. B: daily body weight of vehicle- and CTRP3-injected DIO mice. C: pre- and posttreatment fasting (8 h) blood glucose levels. D: hepatic triglyceride contents in vehicle- and CTRP3-injected DIO mice. E and F: serum triglyceride (E) and ketone (F) levels in vehicle- and CTRP3-injected DIO mice.